Identification of the bound residue composition derived from 14C-labeled chlorsulfuron in soil by using LC-MS and isotop

Abstract： A new method for extracting the bound residue(BR) derived from 14C-labeled chlorsulfuron in soils was developed, and the technique of combining LC-MS with isotope tracing method was subsequently applied to identify the composition of the 14 C-BR in a loamy Fluvent derived from marine deposit. The results showed that the 14C-[2-amino-4-methoxyl-6-methyl-1,3,5]-triazine, 14 C-[ 2-amino-4-hydroxyl-6-methyl-1,3,5]-triazine and 14 C-chlorsulfuron parent compound constituted the main composition of the 14 C-BR derived from 14 C-labeled chlorsulfuron in the soil. The radioactive ratio of three compounds accounted for 39.8 %, 35.4 % and 17.9 % of total recovered radioactivity, respectively. However, a small amount(3.6% of total recovered radioactivity) of the complex of 14 C-[ 2-amino-4-hydroxyl-6-methyl-1,3,5 ]-triazine might have existed in the 14 C-BR in association with an unknown soil substrate. 2-chlorobenzenesulfonamide was also detected to be one of the components of the BR. The results could well explain the mechanism of phytotoxicity caused by the BR derived from chlorsulfuron in soil. In addition, the mechanism of BR formation in soil was also discussed in details. 作 者： YE Qing-fu    WU Jian-min    SUN Jin-he   作者單位： Institute of Nuclear- Agricultural Science, Zhejiang University, Hangzhou 310029, China  期 刊： 環境科學學報(英文版)  ISTICSCI   Journal： JOURNAL OF ENVIRONMENTAL SCIENCES  年，卷(期)： 2004, 16(1)  分類號： X131.3  Keywords： bound residue    chlorsulfuron    composition    identification    soil