Purification and Structure Identification of Hyaluronic Acid

Polysaccharide produced by mutated strain of Streptococcus zooepidemicus was purified by the procedures including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) chromatography and Sephadex G-75 gel filtration. The structure of the purified polysaccharide has been characterized by means of chemical composition analysis, 13C NMR spectrum, infrared spectrum and circular dichroism (CD). All the results showed that the purified polysaccharide was hyaluronic acid (HA). The single helix conformation of the purified HA was determined by Congo red experiment. The molecular weight of the HA was about 1.16×106D, which was measured by viscosity method.

作 者： Shui Sheng HONG Jia Chen Jian Guo ZHANG Yan Chun TAO Lan Ying LIU   作者單位： Shui Sheng HONG,Jia Chen,Lan Ying LIU(College of Life Science,Jilin University,Changchun 130023)

Jian Guo ZHANG(State Key Laboratory of Electroanalytical Chemistry,Chinese Academy of Sciences,Changchun Institute of Applied Chemistry,Changchun 130022)

Yan Chun TAO(Key Laboratory of Supermolecular Structure and Spectroscopy of Educational Ministry,Jilin University,Changchun 130023) 

刊 名： 中國化學快報(英文版)  ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS  年，卷(期)： 2004 15(7)  分類號：   關鍵詞： Streptococcus zooepidemicus   hyaluronic acid   structure identification  

【Purification and Structure Identific】相關文章：

匯報工作：clear and with structure04-25

Competition and monopoly of Chinese rural groundwater market structure and&04-26

A SAPO-11 Silicoaluminophosphate Molecular Sieve with Stable Crystal Structure04-27

Synthesis and Crystal Structure of Binuclear Copper(Ⅱ)Complex Containing (Dimethylaminomethyl)ferrocene04-27

Effects of Ca on the distribution,structure and morphology of aluminum species in polyaluminum chloride04-27

Periodic Structure of Equatorial Envelope Rossby Wave Under Influence of Diabatic Heating04-27

Effects of traffic pollution on the genetic structure of Poa annua L.populations04-26

Design of Neural Network Variable Structure Reentry Control System for Reusable Launch Vehicle04-26

Photosynthesis and Molecular Structure of 2-Amino-3H-phenoxazin-3-one04-27

Static Force Reliability Analysis of Truss Structure with Piezoelectric Patches Affixed to Its Surface04-26